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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
06/09/2021 |
Actualizado : |
25/04/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CLAVIJO, F.; CURLAND, R.D.; CROCE, V.; LAPAZ, M.I.; DILL-MACKY, R.; PEREYRA, S.; SIRI, M.I. |
Afiliación : |
FELIPE CLAVIJO,, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay.; REBECCA D. CURLAND,, University of Minnesota System, 311816, Plant Pathology, Minneapolis, Minnesota, United States.; VALENTINA CROCE, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay.; MARÍA INÉS LAPAZ, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay.; RUTH DILL-MACKY, University of Minnesota System, 311816, Plant Pathology, Minneapolis, Minnesota, United States.; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA INÉS INÉS SIRI, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay. |
Título : |
Genetic and phenotypic characterization of Xanthomonas species pathogenic of wheat in Uruguay. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Phytopathology, March 2022, vol. 112 (3): 511-520. Doi: https://doi.org/10.1094/PHYTO-06-21-0231-R |
ISSN : |
e-ISSN:1943-7684 |
DOI : |
10.1094/PHYTO-06-21-0231-R |
Idioma : |
Inglés |
Notas : |
Article history: Accepted for publication/ Posted online on 12 Aug 2021, First Look. |
Contenido : |
Abstract: Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol using species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis (MLSA) of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing (MLST) was applied to examine the genetic diversity among X. translucens strains. Strains were assigned to four different sequence types, three of which have been previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to Xanthomonas prunicola, and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota, US. In planta pathogenicity assays carried out on a BLS susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, while non-translucens Xanthomonas sp. strains elicited an atypical symptom of dry necrosis. These findings suggest that local wheat fields are affected by X. translucens pv. undulosa, and by a new wheat pathogen within the Xanthomonas genus. MenosAbstract: Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol using species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis (MLSA) of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing (MLST) was applied to examine the genetic diversity among X. translucens strains. Strains were assigned to four different sequence types, three of which have been previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to Xanthomonas prunicola, and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota, US. In planta pathogenicity assays carried out on a BLS susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, while non-translucens... Presentar Todo |
Palabras claves : |
Bacterial leaf streak; Bacterial Pathogens; Multilocus sequence analysis and typing; POPULATION BIOLOGY; WHEAT; Xanthomonas. |
Thesagro : |
FITOPATOLOGIA; TRIGO; TRITICUM AESTIVUM; URUGUAY. |
Asunto categoría : |
H20 Enfermedades de las plantas |
Marc : |
LEADER 02834naa a2200349 a 4500 001 1062388 005 2022-04-25 008 2022 bl uuuu u00u1 u #d 022 $ae-ISSN:1943-7684 024 7 $a10.1094/PHYTO-06-21-0231-R$2DOI 100 1 $aCLAVIJO, F. 245 $aGenetic and phenotypic characterization of Xanthomonas species pathogenic of wheat in Uruguay.$h[electronic resource] 260 $c2022 500 $aArticle history: Accepted for publication/ Posted online on 12 Aug 2021, First Look. 520 $aAbstract: Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol using species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis (MLSA) of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing (MLST) was applied to examine the genetic diversity among X. translucens strains. Strains were assigned to four different sequence types, three of which have been previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to Xanthomonas prunicola, and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota, US. In planta pathogenicity assays carried out on a BLS susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, while non-translucens Xanthomonas sp. strains elicited an atypical symptom of dry necrosis. These findings suggest that local wheat fields are affected by X. translucens pv. undulosa, and by a new wheat pathogen within the Xanthomonas genus. 650 $aFITOPATOLOGIA 650 $aTRIGO 650 $aTRITICUM AESTIVUM 650 $aURUGUAY 653 $aBacterial leaf streak 653 $aBacterial Pathogens 653 $aMultilocus sequence analysis and typing 653 $aPOPULATION BIOLOGY 653 $aWHEAT 653 $aXanthomonas 700 1 $aCURLAND, R.D. 700 1 $aCROCE, V. 700 1 $aLAPAZ, M.I. 700 1 $aDILL-MACKY, R. 700 1 $aPEREYRA, S. 700 1 $aSIRI, M.I. 773 $tPhytopathology, March 2022, vol. 112 (3): 511-520. Doi: https://doi.org/10.1094/PHYTO-06-21-0231-R
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| Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
27/04/2020 |
Actualizado : |
08/02/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
DIEZ VIGNOLA, M.; SAINZ, M.; SALDAIN, N.E.; MARCHESI, C.; BONNECARRERE, V.; DÍAZ GADEA, P. |
Afiliación : |
MANUEL DIEZ VIGNOLA, Facultad de Agronomía, UDELAR UY.; MARTHA SAINZ, Facultad de Agronomía, UDELAR UY.; NÉSTOR ELIO SALDAIN CROCCE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLAUDIA ELIZABETH MARCHESI GYERMAN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PEDRO DÍAZ GADEA, Facultad de Agronomía, UDELAR UY. |
Título : |
Limited induction of ethylene and cyanide synthesis are observed in quinclorac-resistant barnyardgrass (Echinochloa crus-galli) in Uruguay. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Weed Science, 1 July 2020, Volume 68, Issue 4, Pages 348-357. Doi: https://doi.org/10.1017/wsc.2020.32 |
DOI : |
https://doi.org/10.1017/wsc.2020.32 |
Idioma : |
Inglés |
Notas : |
Article history: Article accepted and Published online by Cambridge University Press: 28 April 2020 |
Contenido : |
Abstract: Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv] is the foremost weed in rice (Oryza sativa L.) systems and its control is crucial to successful rice production. Quinclorac, a synthetic auxin herbicide, has been used effectively to manage E. crus-galli. However, the occurrence of quinclorac-resistant genotypes are frequently reported, and its resistance evolution has questioned the continued utility of quinclorac for grass control. Identification of the resistance mechanism(s) of resistant genotypes will facilitate development of integrated weed management strategies that sustain quinclorac use for management of E. crus-galli. We evaluated the responses to quinclorac of two contrasting genotypes: E7 (resistant, R) and LM04 (susceptible, S). Quinclorac induced ethylene and cyanide biosynthesis in the S-genotype. Both genotypes responded similarly to an increasing application of exogenous 1-carboxylic acid aminocyclopropane (ACC) and potassium cyanide (KCN), and their growth was inhibited at higher doses. The key mechanism for cyanide (HCN) detoxification in plants, β-cyanoalanine synthase (β-CAS) activity, was evaluated in both genotypes, and no significant difference was observed in the basal activity. However, quinclorac significantly induced β-CAS-like activity in the S-genotype, which is consistent with the increased synthesis of ethylene and cyanide. This work suggests that the resistance to quinclorac of the E7 R-genotype is likely related to an alteration in the auxin signal transduction pathway, causing a lower stimulation of ACC synthase and, therefore, limited synthesis of ethylene and HCN after quinclorac treatment. MenosAbstract: Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv] is the foremost weed in rice (Oryza sativa L.) systems and its control is crucial to successful rice production. Quinclorac, a synthetic auxin herbicide, has been used effectively to manage E. crus-galli. However, the occurrence of quinclorac-resistant genotypes are frequently reported, and its resistance evolution has questioned the continued utility of quinclorac for grass control. Identification of the resistance mechanism(s) of resistant genotypes will facilitate development of integrated weed management strategies that sustain quinclorac use for management of E. crus-galli. We evaluated the responses to quinclorac of two contrasting genotypes: E7 (resistant, R) and LM04 (susceptible, S). Quinclorac induced ethylene and cyanide biosynthesis in the S-genotype. Both genotypes responded similarly to an increasing application of exogenous 1-carboxylic acid aminocyclopropane (ACC) and potassium cyanide (KCN), and their growth was inhibited at higher doses. The key mechanism for cyanide (HCN) detoxification in plants, β-cyanoalanine synthase (β-CAS) activity, was evaluated in both genotypes, and no significant difference was observed in the basal activity. However, quinclorac significantly induced β-CAS-like activity in the S-genotype, which is consistent with the increased synthesis of ethylene and cyanide. This work suggests that the resistance to quinclorac of the E7 R-genotype is likely related t... Presentar Todo |
Palabras claves : |
?-CYANOALANINE; AUXIN HERBICIDE; BARNYARDGRASS; RECISTANCE; RICE. |
Thesagro : |
ARROZ. |
Asunto categoría : |
H60 Malezas y escardas |
Marc : |
LEADER 02659naa a2200277 a 4500 001 1061037 005 2021-02-08 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1017/wsc.2020.32$2DOI 100 1 $aDIEZ VIGNOLA, M. 245 $aLimited induction of ethylene and cyanide synthesis are observed in quinclorac-resistant barnyardgrass (Echinochloa crus-galli) in Uruguay.$h[electronic resource] 260 $c2020 500 $aArticle history: Article accepted and Published online by Cambridge University Press: 28 April 2020 520 $aAbstract: Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv] is the foremost weed in rice (Oryza sativa L.) systems and its control is crucial to successful rice production. Quinclorac, a synthetic auxin herbicide, has been used effectively to manage E. crus-galli. However, the occurrence of quinclorac-resistant genotypes are frequently reported, and its resistance evolution has questioned the continued utility of quinclorac for grass control. Identification of the resistance mechanism(s) of resistant genotypes will facilitate development of integrated weed management strategies that sustain quinclorac use for management of E. crus-galli. We evaluated the responses to quinclorac of two contrasting genotypes: E7 (resistant, R) and LM04 (susceptible, S). Quinclorac induced ethylene and cyanide biosynthesis in the S-genotype. Both genotypes responded similarly to an increasing application of exogenous 1-carboxylic acid aminocyclopropane (ACC) and potassium cyanide (KCN), and their growth was inhibited at higher doses. The key mechanism for cyanide (HCN) detoxification in plants, β-cyanoalanine synthase (β-CAS) activity, was evaluated in both genotypes, and no significant difference was observed in the basal activity. However, quinclorac significantly induced β-CAS-like activity in the S-genotype, which is consistent with the increased synthesis of ethylene and cyanide. This work suggests that the resistance to quinclorac of the E7 R-genotype is likely related to an alteration in the auxin signal transduction pathway, causing a lower stimulation of ACC synthase and, therefore, limited synthesis of ethylene and HCN after quinclorac treatment. 650 $aARROZ 653 $a?-CYANOALANINE 653 $aAUXIN HERBICIDE 653 $aBARNYARDGRASS 653 $aRECISTANCE 653 $aRICE 700 1 $aSAINZ, M. 700 1 $aSALDAIN, N.E. 700 1 $aMARCHESI, C. 700 1 $aBONNECARRERE, V. 700 1 $aDÍAZ GADEA, P. 773 $tWeed Science, 1 July 2020, Volume 68, Issue 4, Pages 348-357. Doi: https://doi.org/10.1017/wsc.2020.32
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